DEV01236 Supplemental Figures

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Xenopus transgenic embryos expressing zebrafish tbx6 transcripts. Stage 20-23 embryos, anterior towards the left with dorsal towards the top. (A,B) Zebrafish tbx6 transcripts are detected in the posterior paraxial mesoderm of Xenopus transgenic embryos injected with PAC-tbx6 (black arrows). The embryo in B is tilted slightly to show the bilateral expression patterns of the tbx6 transcripts. (C) Xenopus embryo generated from nuclei without the addition of the PAC-tbx6 DNA does not express the tbx6 transcript. (D) Xenopus transgenic embryo injected with PAC-tbx6 shows normal Xbra expression in the paraxial mesoderm, indicating normal development of the posterior mesoderm.

• Supplemental Figure 2 -

  Fig. S2. In situ hybridization of embryos expressing ptbx6-1.7-gfp and ptbx6-2.0-gfp. (A,B) Animal (A) and lateral (B) views of zebrafish embryos injected with ptbx6-1.7-gfp showing strong mosaic expression of gfp in the margin at the shield stage. (C) Lateral view with anterior to the top of a ptbx6-1.7-gfp injected embryo injected at the 15- to 18-somite stage shows strong and specific gfp expression in the tail mesoderm. (D) Higher magnification of C, focusing on the tail region. (E-H) Same as panels A-D, except that ptbx6-2.0-gfp was injected into these embryos.

• Supplemental Figure 3 -

  Fig. S3. Cross-regulation between the wnt8 and zebrafish bmp4 gene expression. (A,B) Animal views with dorsal towards the left of shield stage embryos. Expression of zebrafish bmp4 in an uninjected embryo (A) and embryo injected with the wnt8MOs (B) showing a dorsal reduction in the expression of zebrafish bmp4 in the wnt8 morphant. (C,D) Lateral views. (E,F) Animal views with dorsal to the left of 80% epiboly embryos. Expression of wnt8 in a wild-type embryo (E) and swr embryo (F) showing the expression of wnt8 is only slightly decreased in the swr mutant embryo. (G,H) Lateral views.