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Fig. 5. Expression of duf-lacZ during abdominal myogenesis. Preparations
of duflacZ pupae double labeled with anti-ß-galactosidase
(green) and anti-Twist (red). Panels A and B are of the same preparations
shown separately in the red and green channels. (C-F) Merged images. (A)
Twist-expressing nerve-associated myoblasts in the dorsal hemisegment of A3 in
a late third instar larva. (B) duf-lacZ expression in the same cells
as in A. (C) A 13-hour APF pupa, again showing co-localization of
duf-lacZ with Twist in almost all nerve-associated myoblasts. (D) By
20 hours APF, lacZ expression has faded from many of the myoblasts,
and only a subset of myoblasts shows co-localization. (E) At 24 hours APF, the
number of Twist and duf-lacZ co-localizing myoblasts is further
reduced. By this stage, individual myoblasts expressing high levels of
duf-lacZ are seen. (F) Twenty-eight hours APF. Single
duf-lacZ-expressing myoblasts are observed at positions corresponding
to future fibre forming sites. In all figures anterior is to the top, dorsal
to the right. Scale bar: 20 µm.
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