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Fig. 6. Expression of duf-lacZ in the adult thoracic myoblast pool. (A-C)
Expression of duf-lacZ in the wing disc-associated myoblasts. (A)
Faint duf-lacZ expression in the myoblasts that adhere to the notal
region of the wing disc. The region outlined by the black box is magnified in
B and C. (B,C) Twist-expressing myoblasts (red; B) that also express
duf-lacZ (green; C). (D-F) Co-localization of duf-lacZ with
1151Gal4-expressing myoblasts. (D) Confocal image of a 12-hour APF hemithorax
showing the 1151-GAL4 expressing myoblasts of the developing DVM II fibres.
1151Gal4-driven expression is revealed by UAS-nls-GFP, with duf-lacZ
in the background. Each arrowhead indicates a nucleus, which is larger in size
than the other nuclei within the syncitium. (E) The
duf-lacZ-expressing myoblasts of DVM II, assayed by
anti-ß-galactosidase labeling. These two nuclei are the same as those
indicated by arrowheads (D). (F) Merged image showing the co-localization of
duf-lacZ (red) with 1151GAL4 (green). (G-I) Co-localization of
duf-lacZ with Vg. (G) Confocal image showing Vg-expressing myoblasts
that have fused to, or have swarmed over, the DVM II syncitium. Vg expression
is observed by anti-Vg labeling on a 12-hour APF duflacZ pupa. Amidst
the cluster of myoblasts, arrowheads indicate the myoblasts that also express
duf-lacZ. (H) Anti-ß-galactosidase labeling reveals the
duf-lacZ-expressing myoblasts of DVM II in the same preparation. (I)
Merged image showing the co-localization of Vg with duf-lacZ. Scale
bar: 50 µm for B,C; 30 µm for D-I.
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