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Fig. 2. Expression of Sox9 mRNA and SOX9 protein in 9-day-old (E9) chick GI tract. (A) Whole-mount in situ hybridization of E9 dissected gut using antisense Sox9 riboprobe. Paraffin cross-sections along AP axis as indicated in A, stained with anti-SOX9 (B1,C1,D1,E1,F1) or anti-HNK1 (B2,C2,D2,E2,F2) antibodies. Cross-sections of the gut were done at the following levels: esophagus (B1,B2), pyloric sphincter (C1,C2), midgut anterior to the umbilicus (D1,D2), caeca (E1,E2), hindgut (F1,F2) and cloaca chamber (G1,G2). Note that mesodermal SOX9 expression strongly demarcates the pyloric sphincter (A,C1). SOX9 is strongly expressed in the epithelia of the esophagus, the small intestine and the cloaca chamber (B1,D1,G1), but faintly in the hindgut (F1). Abbreviations: an, anus; cae, caeca; cl, cloaca; eso, esophagus; gizz, gizzard; hg, hindgut; liv, liver; mg, midgut; panc, pancreas; pv, proventriculus; pyl, pyloric sphincter.





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