|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Modulation of the BMP signaling pathway in the stomach affects
Sox9 expression. Whole-mount in situ hybridization using antisense
Sox9 riboprobe on GFP- (A), Bmp4- (B),
Nkx2.5- (C) and Noggin- (D,E) misexpressing E8 stomachs. The
morphological change of Bmp4-misexpressing stomach is characterized
by a reduced musculature of the gizzard (compare B with A). Noggin
misexpression in the stomach gives rise to a range of phenotypes (D,E).
Moderate phenotype present proventriculus fate change with gland formation
inhibition and size increase (D). Severe Noggin phenotype is mainly
characterized by stomach/duodenum connection defect and gizzard-like phenotype
found in the whole stomach (E). Neither GFP (A) as control nor
Nkx2.5 (C) misexpression affects stomach morphology. Sox9
expression is upregulated in Bmp4-misexpressing stomach (B), strongly
downregulated in Noggin-misexpressing stomachs (D,E) and not affected
in Nkx2.5- (C) or GFP- (A) misexpressing stomachs. Red and
black arrows indicate pyloric sphincter area. (F) Bmp4-misexpressing
stomach was sectioned and probed with anti-SOX9 antibodies and revealed SOX9
ectopic expression in the gizzard (red arrowheads, F).
|
