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Fig. 2. Sox2ß-geo expression (X-gal staining, blue) in
neural and forebrain development and in adult neural stem cells from
Sox2ß-geo/+ mice. (A) Undifferentiated ES cells; (B)
E7.5 neural plate stage. Right, anterior; left, posterior. A few blue cells
are also visible at the base of the allantois, where the first appearance of
primordial germ cells has been described. (C) E12.5 embryo; (D-G) coronal
sections of forebrain at E11.5 (D), E12.5 (E) and E17.5 (F,G). In F, asterisks
indicate developing thalamic nuclei, basolateral (more external) and
centromedian and paraventricular (closer to the midline); black circle
indicates the striatum. In G, the black arrowhead indicates the choroid
plexus. GE, ganglionic eminence; LV, lateral ventricle. (H-Q) Adult brain. (H)
Sagittal section with anterior to the left and posterior to the right. Brief
X-gal reaction development. Note the intensely stained ventricle lining and
rostral migratory stream. (I,J) Coronal sections (I is more posterior than J)
after a prolonged X-gal reaction development. Asterisk and black arrowheads,
thalamic nuclei; black circle, dorsal striatum; white circle, septum. (K,L)
Interference contrast microscope view of lateral ventricle wall showing
details from a section corresponding to that shown in J. Black arrow,
ependymal staining; white arrows, subependymal staining. (M) Thalamic region
detail, at a level slightly posterior to that shown in I (and corresponding to
that shown in F; see F, I and J for symbols). (N) Hippocampus dentate gyrus
(long X-gal reaction development). Arrows indicate the germinative layer;
asterisks indicate the granule cell layer. (O) Neurospheres, the progeny of
stem cells, grown in vitro from adult brain lateral ventricles in the presence
of G418 for 5-12 passages (about 5-12 weeks) express lacZ in most
cells. (P,Q) Cells obtained by differentiation of the clonal progeny of a
single G418-resistant (i.e. Sox2 expressing) cell were probed with
antibodies against (P) ß-tubulin III (neurones; red) and GFAP (astroglia;
green), or (Q) GalC (oligodendroglia).
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