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Fig. 4. Double immunofluorescence characterization of Sox2-expressing cells in adult neurogenic regions (by confocal microscopy). Coronal sections from mice treated with BrdU for one week. (A-D'') Sections comprising the lateral ventricle were labelled with antibodies against SOX2 (green, A'-D'), BrdU (red, A'',B'') and GFAP (red, C'',D''). Co-expression of SOX2 and BrdU, or SOX2 and GFAP results in yellow staining in merged images (A-D). (A,C) Top corner of lateral ventricle, comprising the origin (top left of each panel) of the rostral migratory stream. In C, white arrowheads indicate some of the SOX2/GFAP double-positive cells in the rostral migratory region. (B) Detail of the ventricle wall, lining the striatum. (D) Magnification of a detail of the ventricle wall, adjacent to the striatum, showing a subependymal SOX2/GFAP double-positive cell [for the morphology of GFAP-positive cells in a similar region, see Doetsch (Doetsch, 2003)]. (E-G) Sections comprising the basal portion of the hippocampus dentate gyrus. (E) General view of anti-SOX2-labelled dentate gyrus (red). (F) Magnification of a SOX2-positive cell at the basis of the dentate gyrus. (G,H) SOX2/BrdU (G) and SOX2/GFAP (H) double-positive cells are located in the germinative zone at the basis of the dentate gyrus. (G',H') SOX2, green label; (G'') BrdU or (H'') GFAP, red label. (G,H) Yellow indicates double positivity. The position of the cell relative to the germinative cell layer (GCL) is indicated by a dotted white line, which represents the border between the GCL and the hilus (HIL).





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