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Fig. 3. A wave of shh-GFP expression spreads through the GCL and INL
independently of ath5 activity. All images are confocal sections of
the retina, with anterior to the top. (A) Wild-type embryo double labeled for
Zn5 protein (red) and shh-GFP expression (green) at 32 hpf. The
expression of both markers has been initiated in the ventronasal sector. Note
that some of the shh-GFP-expressing cells do not co-express Zn5, and
are therefore amacrine cells (inset, arrowhead). (B) Wild-type embryo double
labeled for Zn5 and shh-GFP at 57 hpf. Expression of both markers has
spread through the retina. (C) ath5 mutant embryo double labeled for
Zn5 (red) and shh-GFP expression (green) at 32 hpf. Note the absence
of Zn5-expressing cells, while a small number of shh-GFP-expressing
cells are already present (arrowheads). (D) ath5 mutant embryo double
labeled for Zn5 and shh-GFP expression at 57 hpf. Note that
shh-GFP expression has spread through both the GCL and the INL.
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