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Fig. 6. Non-autonomous rescue of Mueller glia at 72 hpf by wild-type cells
transplanted into shh mutant embryos. Wild-type cells are labeled
with biotin (blue) and they also carry the shh-GFP transgene (green).
Mueller glia are detected with an antibody recognizing Glutamine Synthetase
(GS, red). (A) Mueller glia are rescued in the immediate vicinity of
shh-expressing wild-type cells. The arrowheads point to shh
mutant cells that express GS. Arrows indicate wild-type donor cells that
express GS. (B) Higher magnification of the image shown in A.
(B'-B''') Single channels of the image shown in B. Arrowheads point
to mutant cells that are rescued. (C) Another example showing non-autonomous
rescue of mutant cells (arrowheads). Note only two shh-GFP-expressing
wild-type cells are present in the INL in the lower portion of the retina, and
that these cells rescue nearby mutant cells. (D) Higher magnification of the
image shown in C. (E) An example of a small clone of wild-type cells
expressing shh-GFP in the neural retina that leads to local rescue of
Mueller glia (arrowheads). (F) A slightly larger clone of wild-type cells
expressing shh-GFP that leads to local rescue of Mueller glia
(arrowheads). (G) An example of failure to rescue by wild-type cells
expressing shh-GFP present in the pigmented retina (RPE, arrowheads).
(Inset) Enlarged view of framed area.
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