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Fig. 7. Non-autonomous rescue of photoreceptors at 72 hpf by wild-type cells
transplanted into shh mutant embryos. Wild-type cells are labeled
with biotin (blue) and they also carry the shh-GFP transgene (green).
Photoreceptors are detected with an antibody recognizing Zpr1 (red). (A)
Photoreceptors are rescued when they are found in the region of the retina
containing wild-type shh-expressing cells in the neural retina. The
arrow indicates shh mutant cells that are rescued. (B) Higher
magnification of the image shown in A. Arrowheads point to non-autonomously
rescued cells. (C) Another example of the rescue of photoreceptors. (D) Higher
magnification of the image shown in C. Arrowheads point to non-autonomously
rescued cells. (E) A further example of rescue. Note that there is a gap in
the rescued photoreceptors in exactly the same region as where there are no
shh-expressing cells in the neural retina (arrow). (F) Example of
failure to rescue photoreceptors when wild-type cells are located only in the
RPE (arrowheads). (Inset) Enlarged view of framed area.
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