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Fig. 8. Non-autonomous rescue of lamination at 50 hpf by wild-type cells
transplanted into shh mutant embryos. Wild-type cells are unlabeled,
but they carry the shh-GFP transgene (green). The inner plexiform
layer is detected with phalloidin staining (red). (A) An example of a very
small group of wild-type shh-expressing cells that lead to elevated
levels of F-actin in adjacent cells (arrowhead) in the position where the IPL
would normally form. (B) Higher magnification of the image in A. (C) Two
groups of wild-type shh- expressing cells lead to local rescue of the
IPL (arrowheads). (D) Higher magnification of the image in C. (E-G). Local
rescue of the IPL by groups of wild-type cells expressing shh. In G,
wild-type cells are labeled in blue, but rescue is only observed close to
shh-GFP-expressing wild-type cells (arrowhead). (H) Large clones
(blue) fail to rescue lamination if they do not contain shh-GFP
expressing cells (green). (I) Wild-type cells expressing shh-GFP in
the RPE fail to rescue lamination. (I') The GFP single channel of the
image shown in I, showing shh-GFP-expressing cells in the RPE
(arrowhead, inset).
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