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Fig. 1. Expression array analysis of E13.5 trigeminal ganglia. (A) A
two-dimensional plot of the hybridization signal for 
4,000 present
transcripts, including ESTs, in one of the two experiments comparing
Brn3a heterozygous and knockout trigeminal ganglia. Values for the
expression of the majority of transcripts fall along the central diagonal line
representing equal expression in the two genotypes. A few transcripts fall
outside the parallel lines indicating a greater than twofold change in
expression, and examples of some highly changed transcripts are indicated. (B)
To determine whether Brn3a heterozygosity results in intermediate
levels of target gene expression, two-way comparisons were made between
transcript levels in knockout versus wild-type, knockout versus heterozygous
and wild-type versus heterozygous ganglia for the 41 increased and 62
decreased transcripts from the U74Av2 array, Experiment 1. Expression levels
in wild type and heterozygotes showed a similar mean fold increase or decrease
when compared to those in the knockout. However, comparison of expression
levels in heterozygotes with those in wild type revealed no significant
difference (fold change 1, red horizontal line) for either the increased
or decreased transcripts. As expected, the fold change was also approximately
1 for two-way comparisons between the genotypes for a group of 160 unchanged
neural transcripts (0.003<change-p<0.997; see Supplemental Data,
http://dev.biologists.org/supplemental/).
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