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Fig. 1. Targeted deletion of the ß1-integrin gene in NCC derivatives. (A) The
lower panel summarises the NCC lineage and its derivatives (at trunk level),
showing that the floxed ß1-integrin gene is recombined in the neurons and
SC of the sensory nerves and only in the SC of the motor nerves of the PNS.
The upper panel depicts the genomic structure of the floxed ß1-integrin
gene allele before and after Cre-dependent DNA recombination. Following
recombination, the lacZ reporter gene is expressed under the control
of the endogenous ß1-integrin gene promoter (ß1 pr). (B) Section
(200 µm) of a control embryo
(Ht-PA-Cre;ß1fl/ß1+), showing the distribution
of ß-galactosidase activity on E13. The entire PNS is stained, including
the spinal nerve and enteric nervous system (white arrowhead). (C,D)
ß1-integrin immunolocalisation in sagittal sections of an E10 mutant
embryo (C; Ht-PA-Cre;ß1fl/ß1–) and a
control embryo (D) at the level of the branchial arches. (E-I)
ß1-integrin immunodetection in transverse sections, made at the level of
the DRG, of E12.5 (E,F) and E13 (G-I) embryos with mutant (E and G,
respectively), control (F and I, respectively) or heterozygous (H;
Ht-PA-Cre;ß1–/ß1+) genotype. The white
arrowheads and arrows indicate the structures derived and not derived from
NCC, respectively. The loss of ß1-integrins in the NCC-derived structure
of DRG is achieved at E13. DRG, dorsal root ganglia; SG, sympathetic ganglia;
NT, neural tube. Scale bars: 50 µm.
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