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Fig. 4. Morphology of the spinal nerves in control and mutant embryos, on E12.5 and
E13.5. (A-H) ß-Galactosidase activity was detected in whole-mount
preparations. Lateral view of control embryo (A) showing intense labelling of
craniofacial structures and PNS. The mutant (B) displays an absence of
labelling in the trunk PNS. The 200 µm transverse sections of whole-mount
E13.5 control (C) and mutant (D) embryos reveal the loss of reporter gene
expression in the distal part of the mutant innervation network (white arrows
in D). (E-H) Detail of cutaneous lateral ramus and lateral muscular
innervation. The immunolocalisation of NF-160 on similar 200 µm transverse
sections from control (E,F) and mutant (G,H) embryos shows the presence of
nerves in both cases. However, SC have been specifically lost from the distal
part of the mutant nerves (black arrowhead in G,H).
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