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Fig. 5. DAF-16 negatively regulates the expression of daf-15. (A) Semi-quantitative RT-PCR shows that expression of daf-15 is negatively regulated by DAF-16. The control rpl-21 gene (encoding the large ribosomal subunit L21 protein) was equally represented in both RNA preparations. The experiment was performed three times using three independent RNA preparations. (B) EMSA indicates DAF-16 binds specifically to the daf-15 IRS in vitro (see text).





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