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Fig. 1. C57BLKS Bst/+ phenotypes. (A) Kinked tail (arrowhead), white hind feet and belly spot. (B) Hematoxylin and eosin staining of +/+ and Bst/+ retinal sections. (C) Neurofilament (NF160) immunostaining of adjacent sections at the optic nerve head, marking ganglion and horizontal cells. (D) Skeletal stain of newborn fore limbs (upper) and hind limbs (lower), showing preaxial polydactyly (0) and triphalangy of the first digit (1) in Bst/+ limbs. (E) Postnatal growth curves of littermate +/+ and Bst/+ pups. (F) Weights (grams±s.e.m.) of adult +/+ and Bst/+ littermates, compared using a two-tailed unpaired t-test (n=44 total). Scale bars in B,C: 50 µm. ON, optic nerve head; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.





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