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Fig. 2. Scanning electron and light micrographs illustrating epidermal and histological features of wild-type and mutant leaves. (A-C) SEM of adaxial surface of wild-type (A) blade, (B) auricle and (C) sheath. (D-G) Transverse section through wild-type (D) blade, (E) auricle, (F) internal sheath and (G) marginal sheath tissue. (H) Cartoon depicting regions where tissue was sampled. (I-K) Transverse sections through (I) ectopic auricle in Wab1 blade, (J) ectopic sheath in Wab1 blade and (K) ectopic sheath in lg1-R;Wab1 blade. (L-M) SEM of adaxial surface of (L) Wab1 ectopic auricle and (M) lg1-R;Wab1 ectopic sheath. All sections are oriented with the adaxial surface upwards. Arrows in A and D indicate multicellular base of macrohair. Normal bundle-sheath anatomy indicated by arrowhead in D, abnormal bundle-sheath anatomy indicated by arrowheads in I and J. Scale bars: 100 µm.





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