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Fig. 4. Sector phenotypes in Wab1 mutants. (A-E) Abaxial view of
Wab1 leaves with auricle extension and/or ectopic auricle phenotypes
with albino sectors exhibiting normal blade characteristics. (F) Scanning
electron micrograph of adaxial surface of boxed region in C, illustrating
unexpanded, dovetailed auricle-like cells in Wab1/wab1+ ectopic
auricle tissue (right of arrowhead), and normal blade epidermal
characteristics such as macrohairs in the wab1+/– sector (left
of arrowhead). (G) Fluorescence micrograph of a transverse section through a
sector adjacent to auricle extension, illustrating a sharp boundary between
green, Wab1/wab1+ auricle-like tissue (which fluoresces red), and
albino, wab1+/– tissue (which appears blue-green). (H) SEM of
adaxial surface of sector boundary near that shown in G, with hairy, fully
expanded auricle-like cells in the Wab1/wab1+ tissue (right of
arrowhead) and normal blade cells including prickle hairs and macrohairs in
the wab1+/– tissue (left of arrowhead). (I) Fluorescence
micrograph of a transverse section through the sector shown in J. Green,
Wab1/wab1+ tissue is very thin with widely spaced veins, a hairless
adaxial surface and abaxial hairs characteristic of marginal sheath tissue.
Albino wab1+/– tissue has the histological organization of
normal blade tissue. (J) Abaxial view of sector adjacent to ectopic sheath
tissue, arrowhead marks sector boundary. (K) Cartoon depicting an albino
non-mutant (w3 wab1+/–) sector in a green Wab1/wab1+
leaf. Scale bar: 500 µm (F) and 100 µm (G-I).
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