spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. A transcriptional enhancer from Mef2c is sufficient to direct expression to the anterior heart field in vivo. A 6142 bp fragment of the mouse Mef2c gene was cloned into the lacZ reporter plasmid HSP68-lacZ and used to generate transgenic mice. Representative X-gal stained, transgenic embryos are shown at 7.5 days post coitum (dpc) (A), 8.0 dpc (B), 9.5 dpc (C,D), and 11.5 dpc (F,G). X-gal stained hearts are shown from a transgenic fetus collected at 16.5 dpc (I) and a transgenic adult dissected at 12 weeks of age (J). (E,H) Transverse sections of transgenic embryos collected and X-gal stained at 9.5 dpc and 11.5 dpc, respectively. No expression was observed outside the anterior heart field at any stage. (A) Parafrontal view; (B,C,F) lateral views from the right; (D,G,I,J) ventral views. BA, branchial arches; LA, left atrium; LV, left ventricle; NT, neural tube; OFT, outflow tract; PA, pulmonary artery; RA, right atrium; RV, right ventricle. Arrowheads denote expression in the pharyngeal mesoderm/anterior heart field. Five independent transgenic lines all displayed nearly identical patterns of expression.





Right arrow Return to article