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Fig. 3. Ectopic eyes on the antenna depend on the C terminus of EY. (A) Column 1 shows the head region, including the antenna of flies carrying the dpp^blink-Gal4 transgene and the corresponding transgene (marked on the left of each row) in a wild-type background. Ectopic eyes (arrows) on the antenna were induced only by transgenes containing the EY C terminus. Column 2 shows so10-lacZ expression on eye-antennal discs of third instar larval stage. In addition to the so10-lacZ transgene, they express the same transgenes as the flies in column 1. ß-Galactosidase expression was detected only in antennal discs where ectopic eyes on the adult antenna were also observed. Asterisks mark the area of ectopic staining. (B) Western blot analysis of third instar leg discs carrying the so10-lacZ, the dpp^blink-Gal4 transgenes and the different ey/toy chimeras in a wild-type background. Molecular weight marker is indicated at the right. The western blot was probed once with a rabbit -quail-PD antibody (upper) and once with an anti-ß-Galactosidase antibody (lower). The various Pax6 proteins are indicated by an asterisk. (Lanes 1-6) Misexpression of toy (lane 1), ey (lane 2), toyPD-EYBB (lane 3), EYBB-toyCT (lane 4), TOYBB-eyCT (lane 5) and eyPD-TOYBB (lane 6). Lanes 3 and 5 show a higher amount of ß-Galalactosidase protein, consistent with the fact that both transgenes inducing ß-Galalactosidase contain the ey C terminus.

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