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Fig. 6. Repression of antennal identity by the EY-HD. (A-F) Flies carrying the ey-Gal4, the UAS-P35 and the UAS-ey (A,D), the UAS-ey{Delta}PD (B,E) or the UAS-ey{Delta}HD (C,F) transgenes in an ey2 mutant background. A and C show a full rescue of the ey2 mutant eye phenotype by a PD containing EY protein. The percentage of flies rescued by ey or ey{Delta}HD are 50% or 79%, respectively, for a rescue of at least 80% of the wild-type eye size (Punzo et al., 2001). D and F show the cases where the rescue did not work at all, even though the PD was present. In those cases, the co-expression of P35 led to a duplication of the antenna (arrowhead, duplicated antenna; arrow, wild-type antenna) only in the absence of the EY-HD, indicating that the HD is able to repress antennal identity. B and E show two different eye sizes of an ey2 mutant, where no rescue is observed because of the expression of a PD-deleted EY protein, but, in addition, no duplication of the antenna is observed because of the presence of the HD.





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