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Fig. 1. Sra-1 binds Kette through its C terminus. (A) Whole-mount stage 16 embryos
stained for kette or sra-1 RNA expression as indicated,
(Left) Lateral views; (middle) ventral views; (right) higher magnifications of
the ventral nerve cord showing ubiquitous expression of both genes. (B)
Western blot analyses of Kette (120 kDa) and Sra-1 (140 kDa) expression. Both
proteins are expressed throughout embryogenesis and appear to be provided
maternally. (C) Co-immunoprecipitation of Kette and Sra-1. Total cell lysate
of S2-cells expressing both Myc-tagged Kette and Sra-1 was subjected to
immunoprecipitation with monoclonal anti-Myc (9E10) or anti-Slit (E555.6D).
Sra-1 is detected only in the anti-Myc precipitate, indicating binding of
Kette and Sra-1. (D) To test the interaction between Sra-1 and Kette, we
turned to a yeast two hybrid assay. Interaction between Sra-1 and Kette
results in growth of the yeast cells and a blue color. Interaction is seen
only when the C terminus is intact (E).
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