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Fig. 3. sra-1 is required for axonal growth. Confocal images of mount
preparations of third instar eye discs (ed) for Sra-1 expression (green) and
the axonal marker 24B10 (red). The merge channel is shown in the bottom row.
(A) In wild type, photoreceptor neurons express both Sra-1 and 24B10, and
project their axons through the optic stalk (os) to the lamina (la) or medulla
(me). (B) Following expression of sra-1 dsRNA using an eyeless-GAL4
driver, Sra-1 protein expression was removed from the eye disc but not the
brain (star). Concomitantly, we observed severe axonal targeting defects. Most
axons appear to be able to enter the brain, but fail to grow towards their
correct targets (arrow). (C,D) Adult eyes of (C) a wild-type fly and (D) a
transgenic fly expressing sra-1 RNAi in the eyeless
pattern.
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