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Fig. 7. Sra-1 affects F-actin formation. During pupal development, bristle
morphology is prefigured by an apical F-actin extension, which can be
visualized by phalloidin staining. All pupae were dissected 36 hours after
puparium formation. (A) Wild type. (B) After expression of sra-1,
dsRNA expression (one copy) using sca-GAL4 bent F-actin bundles, which are
never seen in wild type, can be detected. (C) After expression of one copy of
the sra-1
CMyr construct, no F-actin forms at the site where bristles
are expected to develop (star). (D) Following expression of full-length
membrane tethered Sra-1 protein using the sca-GAL4 driver, F-actin formation
is initiated in a broader region of the apical cell surface (arrow). In
addition, the F-actin at the cell boundary appears to have a `fuzzier'
organization (arrowheads).
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