|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. The wnt8– expanded organizer phenotype is due to
reduced vent and vox expression. (A) Rescue of wnt8
mutants by Vox. gsc expression (bracket) in wild-type (a,c) or
wnt8– (b,d) embryos, uninjected (a,b) or injected
(c,d) with a vox expression plasmid. Some isolated lateral cells
still express gsc in injected wnt8– embryos
because of the mosaic expression of Vox (panel d, arrow). Embryos shown are at
70% epiboly, dorsal view. (B) Reduction of Vent/Vox enhances the
wnt8– organizer phenotype. Graph shows the
percentage of embryos belonging to a specific phenotypic class. Class I,
wild-type chd expression; class II to IV, increasingly expanded
chd expression. 100% of wild-type and wnt8–
embryos belong to class I and class II, respectively. Upon injection of
vent+vox MOs, most wild-type embryos belong to class II
(96.5%, n=85), whereas wnt8– embryos belong
to both classes III and IV (60.7% and 39.3%, respectively, n=28).
Embryos shown at the bottom of the graph are at shield stage, animal view,
dorsal right.
|
