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Fig. 2. Southern blot and phylogenetic tree. (A) Southern blot probed with the full-length kringelchen cDNA. Genomic DNA (10 µg) was digested to completion with HindIII (lane 1), NcoI (lane 2), EcoRV (lane 3) and XhoI (lane 4). HindIII, NcoI and EcoRV cut once within the coding sequence (positions 2076, 1383 and 101 of the full-length cDNA, respectively); XhoI does not cut. The size was determined using a {lambda}/HindIII digested DNA size marker. (B) Phylogeny of class III, IV, V and XII receptor tyrosine kinases (Grassot et al., 2003). Kinase domain sequences from RTK Class III (CSFR/PDGFR), IV (FGFR), V (VEGFR) and XII (Ret) were used for phylogeny inference (distance method). Class IX (Tie) sequences were used as outgroup. Node with bootstrap values over 50 are indicated (500 bootstrap replicates). Dm, Drosophila melanogaster; Hs, Homo sapiens; Hro, Halocynthia roretzi; Cin, Ciona intestinalis; btl, Drosophila breathless FGFR; htl, Drosophila heartless FGFR. Kringelchen branches at the base of the FGFR tyrosine kinase superfamily.





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