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Fig. 5. Inhibition of bud detachment and kringelchen expression after treatment with either the FGFR inhibitor SU5402 or phosphorothioated oligonucleotides. (A) Treatment scheme for SU5402. Buds were treated for 24 hours with SU5402 and examined at stages 3, 5 and 7. Asterisk indicates when in situ hybridization was carried out. (B) Scheme of the increasingly severe abnormalities obtained after treatment with SU5402. (C) Effects of SU5402 treatment on the characteristics of the animals at three different stages after treatment. The most severe abnormality predominated at earlier stages after treatment with either SU5402 or phosphorothioate antisense oligonucleotides. (D-F) In situ hybridization of the kringelchen antisense RNA probe to Hydra with increasingly severe abnormalities produced by SU5402 treatment. (G) Effects of electroporation of PT-antisense oligo 1 in stage 3 buds. Sixty polyps were electroporated, the 39 survivors evaluated. (H) In situ hybridization of kringelchen RNA probe to a typical abnormally elongated bud obtained by the antisense oligonucleotide treatment. Evaluation of the abnormalities and in situ hybridization were carried out 7 days after the end of treatment.





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