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Fig. 2. Gsh2 mutants exhibit reduced retinoid production in the ventral telencephalon. (A-C) Representative pictures of X-gal-positive F9 cells, stably transfected with RARE-lacZ, that were co-cultured for 24 hours with E12.5 LGE explants from wild-type embryos (A), Gsh2 heterozygotes (het, B) or Gsh2 homozygous mutants (KO, C). (D,E) Bar graphs showing the reduced numbers of X-gal-positive cells from LGE (black bars) or MGE (white bars) explants at E12.5 (D), and LGE (black bars) or cortex (white bars) at E16.5 (E). *P<0.05, **P<0.01, one-way ANOVA using a Fisher test as a post-hoc. E12.5, n=4 WT, 12 +/– and 6 –/–; E16.5, n=3 WT, 15 +/– and 5 –/–.





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