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Fig. 2. The induction of Shh and its targets at the ventral forebrain is delayed or attenuated at E8.5. Whole-mount in-situ hybridization of Shh (A-D); Nkx2.1(E-H); Nkx2.2 (I-L); Ptch1 (M-P) of E8.5 dpc wild-type and mutant embryos as indicated. Arrow in (A) indicates the Shh expression in the axial mesoderm notochord. Arrowhead in (B) indicates expression in the ventral forebrain.





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