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Fig. 3. Expression patterns of PTL assessed using RT-PCR. All tissues were
from the Columbia ecotype except lane 11 (Ler). The same total RNA
samples were used for three reactions in each case, using mRNA specific
primers for either PTL (top), flower-specific APETALA3
amplified using essentially the same conditions (centre), or ACTIN2
as a control (bottom). 3.5 µl of the reaction was loaded for PTL
and AP3 reactions, 1.5 µl for the ACT2 control. In lane
6, the receptacle at the base of the siliques was removed before RNA
extraction.
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