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Fig. 4. Expression patterns of PTL using in situ hybridization and GUS
reporter genes. (A) Location of DIG-labelled antisense PTL RNA
(brown) hybridized in situ with PTL mRNA in transverse section of an
inflorescence meristem. Label is concentrated in inter-sepal zones (arrows)
and sepal margins. (The black deposit centred between the two arrows is a
staining artefact.) M, shoot apical meristem; 4, 5 and 6, bud stages. (B-N)
Location of GUS reporter gene product in wholemounts (blue) or in sections
(pink, dark field). (B) Side view of young inflorescences showing four spots
of staining in young flower primordia (p8.0i::GUS). (C) Transverse section of
inflorescence showing absence of early staining in the flower primordium
without the intron, although sepal margin expression still occurs (p8.0::GUS).
(D-H). Five serial transverse sections of an inflorescence showing staining
patterns in buds from stage 1 to 6 (indicated in D). Staining is also present
in the edges of cauline leaves (cl) (p2.0i::GUS). (I,J). Petal primordia (p)
are not stained at stage 6 (I) or 7 (J). ls, lateral stamen (p2.0i::GUS). (K)
Longitudinal section of stage 9 bud showing staining in the basal margins of a
developing petal (p2.0i::GUS). (L) Wholemount of a stamen dissected from a
stage 8 flower, showing lateral staining where developing anther and filament
adjoin (arrows) (p2.0i::GUS). (M) Young seedling viewed from above showing
staining in edges of developing leaves, initially all round, but later limited
to basal regions (p2.0i::GUS). (N) Transverse section of shoot apical meristem
of young seedling showing expression in leaf margins (arrows) and stipules
(asterisks), but none in the shoot apical meristem (M) (p2.0i::GUS). Scale
bars: A,C,L,N, 100 µm; B,M, 500 µm; in D, 500 µm for D-H; I-K, 50
µm.
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