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Fig. 5. Consequences of overexpression and ectopic expression of PTL. (A)
ptl-1 mutant inflorescence. (B) Complementation of ptl-1
mutant phenotype in pPTL(1.3i)>>PTL plant (moderate driver line #5). (C)
Gain-of-function phenotype in pPTL(1.3i)>>PTL ptl-1 plant
(strong driver line #7). Lateral sepals are absent, and four petals are
restored, either separate (SEM inset), or fused along their lateral margins.
(D,E) SEMs of inflorescence apex of (D) wild-type plant and (E)
gain-of-function pPTL(1.3i)>>PTL ptl-1 plant (strong driver
line #1). Buds at stages 4, 5 and 6 are indicated. Lateral sepals are either
absent or narrow in gain-of-function plants (E, arrows). (F) SEM of
pAP1>>PTL plant with inflorescences producing flowers arrested at stage
2. (G) pAP3>>PTL flower showing absence of petals and stamens, and
reduced lateral sepal. (H) SEM of pAP3>>PTL flower showing absence of
petal and stamen primordia in the second and third whorls. ca, carpel; f,
filamentous structure; se, sepal. (I) p35S>>PTL seedling showing final
extent of growth. Strongly inducible PTL target line #10 was used for all
ectopic expression plants (F-I). Scale bars: A-C,G, 1 mm; inset in C, D-F,H,I,
100 µm.
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