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Fig. 2. mRNA is affected in mutant embryos. (A) RT-PCR on cDNA from mutant and
wild-type hi3308 embryos with a pair of hi3308/seahorse
specific primers flanking the insertion site. (B) Control RT-PCR with a pair
of actin specific primers. A serial dilution of cDNA was used to show that
similar amounts of cDNA were used. m, 1 kb ladder; wt, wild type; mu, mutant;
1-4, 1:10, 1:200, 1:4,000 and 1:80,000 dilutions.
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