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Fig. 3. Abnormal gonadal morphology in both male and female Pod1 mutants.
(A) At 12.5 dpc, gonads from XY Pod1 KO mice (bottom) were smaller
than in the wild-type testes (top). (B) Gonads from a Pod1 KO XX
embryo at 12.5 dpc also were reduced in size compared with the control. In
both XY and XX Pod1 KO embryos, an abnormal invagination of the
surface was observed at the anterior region of the gonad (g) (arrowheads). g,
gonad; m, mesonephros. (C-F) Semi-thin sections from 13.5 dpc gonads were
stained with Toluidine Blue. At 13.5 dpc, the wild-type XY gonad (C) showed
organized testicular cords (tc), peritubular myoid cells (pm) and extensive
mesenchyme (m). However, no organized testicular cords, peritubular myoid
cells or mesenchyme were seen in Pod1 KO XY gonads (E). At 13.5 dpc,
Pod1 KO XX gonads (F) showed a similar arrangement of germ (g) and
somatic (s) cells as the control (D), but lacked the small but distinct
mesenchymal zone near the coelomic epithelium. tc, testicular cord; pm,
peritubular myoid cell; m, mesenchyme; ce, coelomic epithelium; g, germ cell;
s, somatic cell.
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