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Fig. 1. BMP4 inhibits oligodendrocyte (OL) lineage commitment by neural progenitor
cells. (A) EGF-generated neurosphere cells from E17 mice (A,B) were grown for
3 days in 10 ng/ml each of NT3, PDGF-AA and EGF. Cells were then dissociated
and plated in media containing 2 ng/ml EGF in absence (A, parts a-c) or
presence of 20 ng/ml BMP4 (A, parts d-f). Cells were stained for CNPase (A,
parts a,d), MBP (A, parts b,e) and GFAP (A, parts c,f). (B) Cells were
immunostained with OLIG1, NG2, CNPase, MBP, GFAP, and ß-tubulinIII, and
the mean numbers of immunoreactive cells were determined. BMP4 treatment
significantly reduced the number of all OL species while increasing the number
of astrocytes. Scale bars represent means±s.e.m. of four independent
experiments. Anova was done to determine significance
(*P<0.05).
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