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Fig. 2. BMP4 decreases expression of OL transcription factors and increases
expression of ID genes. (A) Expression of mRNAs for Olig1, Olig2,
Nkx2.2 and Sox10 was analyzed by quantitative RT-PCR performed
with mRNA extracted from E17 progenitor cells grown in absence (blue) or
presence (red) of 20 ng/ml BMP4 for 2, 8 and 35 hours. BMP4 treatment did not
reduce levels of the transcripts until 8 hours. However, by 35 hours all
transcripts were reduced by as much as 60-fold. (B) Expression of Id2
and Id4 mRNAs was analyzed by quantitative RT-PCR performed with mRNA
extracted from E17 progenitor cells grown in absence (blue) or presence (red)
of 20 ng/ml BMP4 for 2, 8 and 24 hours. Id4 and Id2
transcripts were both induced by BMP4 at 8 hours and subsequent time points.
Graphs shown are representative of experiments done in duplicate three times.
(C) Western blot analyses were performed to detect ID1-4 proteins using cell
lysates from E17 progenitor cells grown in the absence (control) or presence
of BMP4 for 12 hours. BMP4 treatment increased the expression of all the ID
proteins.
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