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Fig. 3. BMP4 acts via ID4 and ID2 to inhibit oligodendrogenesis. (A) Western blot
analyses of ID2 and ID4 proteins was done in E17 progenitor cells infected
with lentivirus containing empty vector (lenti-gfp) or Id2 or
Id4 (lenti-Id). (B) E17 progenitor cells were infected for 2 days
with lentivirus containing empty vector (parts a-f), Id2 (parts g-i),
Id4 (parts j-l) or Id2+Id4 (parts m-o). Cells
infected with empty vector were treated with 20 ng/ml BMP4 in parts d-f.
Immunofluorescence was done for CNPase (parts a,d,g,j,m), MBP (parts
b,e,h,k,n) and GFAP (parts c,f,i,l,o). DAPI was used to stain the nuclei. (C)
Quantification for CNPase, MBP, GFAP and ß-tubulinIII shows that ID
overexpression has effects similar to those of BMP4 treatment, i.e. reduced
numbers of OLs and increased numbers of astrocytes. Scale bars represent
means±s.e.m. of four independent experiments. ANOVA was done to
determine significance (P<0.05). The control group (vector alone)
differed significantly from other groups in numbers of CNPase, MBP and GFAP
immunoreactive cells. There were no significant differences among
ß-tubulinIII groups.
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