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Fig. 6. OLIG proteins co-localize and interact with ID2 and ID4 in neural
progenitor cells. (A) E17 neural progenitor cells were grown for 7 days in the
presence of 10 ng/ml EGF (parts a,d), 3 days with 10 ng/ml PDGF-AA+NT3+EGF
(parts b,e) and 12 hours with 20 ng/ml BMP4 (parts c,f-h). Cells were plated
and stained for OLIG1 and ID2 (part g), OLIG1 and ID4 (part h), OLIG2 and ID2
(parts a-c) and OLIG2 and ID4 (parts d-f). Nuclei were counterstained with
DAPI. In the absence of BMP4, both OLIG1 and OLIG2 were localized primarily in
the nucleus. However, after BMP4 treatment both OLIG1 and OLIG2 were
colocalized with ID2 and ID4 in the cytoplasm. (B,C) Co-immunoprecipitations
were done with protein lysates from cultured E17 neural progenitor cells
treated with BMP4 for 12 hours. OLIG1 (B) and OLIG2 (C) interact with both ID2
and ID4.
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