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Fig. 3. Axonal architecture following lineage-specific ablations. Each panel shows
the medial region of two (E) or three (A-D) midbody segments immunostained for
ACT to reveal axon patterning (red). (A-D) Germinal plates from embryos in
which one teloblast of a given pair was injected with lineage tracer (green;
left side) and its contralateral homolog was ablated (right side). (A)
Unilateral M lineage ablation resulted in a random array of axon fibers. (B)
Unilateral N lineage ablation resulted in coalescence of the PP and MA
segmental nerves at the ganglionic margin (arrows). The ipsilateral connective
was often shifted towards the midline and appeared smaller in diameter
(arrowheads). (C) Unilateral O lineage ablations resulted in a truncated
and/or displaced PP segmental nerve (arrows) on the experimental side relative
to the contralateral control at the same clonal age. (D) Unilateral P lineage
ablations had little effect other than a decrease in diameter and moderate
displacement of the medial end of the MA segmental nerve (arrows). The
transient, lateral longitudinal nerve failed to appear (not shown). (E) A
similar preparation in which O, P and Q lineages are labeled on the left side
and missing from the right side, while both N lineages were labeled with a
different lineage tracer (blue). Unilateral OPQ ablations resulted in
abnormalities of all the segmental nerves ranging from displacement to
reduction in size and/or length, but all nerves could be recognized. Anterior
is upwards. Scale bar: 25 µm.
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