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Fig. 6. Effects of specific N lineage ablations on PP nerve patterning. Each panel shows a right hemiganglion (~80 hours clonal age) from a preparation in which the N lineage was labeled with FDA (green) and axons were labeled by ACT immunostaining (red). The PP nerve is indicated by an arrow in each panel. (A) An unablated control preparation shows the normal, posterior origin of the PP nerve and its association with peripheral nz neurons (arrowhead). (B) Ablation of an ns primary blast cell resulted in a significant reduction in size of the anterior region of the ganglion (broken outline approximates the normal contour), but the PP nerve root maintained its normal position. (C) Ablation of an nf primary blast cell resulted in a significant reduction in size of the posterior region of the ganglion (broken line shows the normal contour). The putative PP nerve branched from the MA nerve at the margin of the ganglion. Anterior is upwards. Scale bar: 25 µm.





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