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Fig. 6. Effects of specific N lineage ablations on PP nerve patterning. Each panel
shows a right hemiganglion (
80 hours clonal age) from a preparation in
which the N lineage was labeled with FDA (green) and axons were labeled by ACT
immunostaining (red). The PP nerve is indicated by an arrow in each panel. (A)
An unablated control preparation shows the normal, posterior origin of the PP
nerve and its association with peripheral nz neurons (arrowhead). (B) Ablation
of an ns primary blast cell resulted in a significant reduction in size of the
anterior region of the ganglion (broken outline approximates the normal
contour), but the PP nerve root maintained its normal position. (C) Ablation
of an nf primary blast cell resulted in a significant reduction in size of the
posterior region of the ganglion (broken line shows the normal contour). The
putative PP nerve branched from the MA nerve at the margin of the ganglion.
Anterior is upwards. Scale bar: 25 µm.