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Fig. 5. Bmp7 induces the formation of epithelial branches in explant culture but does not suppress Fgf10-mediated epithelial bud elongation. (A) E15.5 whole lacrimal gland explants treated with 100 ng/ml Bmp7 for 48 hours contained more epithelial branches than control explants. The error bars are standard errors. The significance value of P<0.05 is calculated according to the Mann-Whitney U test. (B) Left column, epithelial explants adjacent to Fgf10-soaked beads at t=0 hours; right column, the same explants at t=48 hours. Epithelial explants treated with Bmp7 resemble control cultures, whereas those treated with Bmp4 show suppression of the epithelial growth and elongation that is normally observed in response to Fgf10.





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