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Fig. 7. Bmp7-null lacrimal glands show reduced cadherin and SMA expression as well as reduced proliferation. Sections of wild-type (A,C,E) and Bmp7^m1Rob/m1Rob (B,D,F) Day of birth lacrimal glands. (A,B) Pax6 (red) and pan-cadherin (green) immunolabeling. (C-F) Pax6 (red) and SMA (green) immunolabeling. Comparing A with B, it can be seen that the level of cadherin immunoreactivity is greatly reduced in the absence of Bmp7. (C,D) SMA-positive cells (white arrowheads) are fewer in number and stain at lower intensity in the absence of Bmp7. (E,F) Higher magnification of wild-type (E) and Bmp7^m1Rob/m1Rob sections, showing reduced number of SMA-positive cells and their reduced labeling intensity. (G) Graphs showing that, compared with wild-type (red bars), the Bmp7^m1Rob/m1Rob lacrimal gland (blue bars) shows a reduced number of Ki67 immunoreactive cells in both mesenchyme and epithelium. Error bars indicate s.e.m. and significance values are calculated according to Student's t-test. Day of birth glands were analyzed. (H,I) In situ hybridization performed on lacrimal glands from wild-type (H) and Bmp7^m1Rob/m1Rob (I) E17.5 embryos using a probe for Fgf10.

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