|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Deletions within residues 616-618 of Vas impair eIF5B-interaction. (A)
Specific Vas deletions, which were transformed into yeast strain EGY48, were
tested for their level of protein expression in yeast on a western blot probed
with anti-Vas. As a loading control, the blot was also probed with anti-Actin
(ICN Biomedicals). (B) Vas deletions were tested for interaction with eIF5B in
the yeast two-hybrid system using a liquid assay for ß-galactosidase
activity. The positive control for interaction is the full-length Vas protein
(1-661). The value shown for each sample represents the average of three
replicates, and the error bars represent s.d. (C) Vas deletions were also
tested for interaction with Osk, using the same system.
|
