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Fig. 3. Localization and expression of transgenic GFP-fusion proteins. For each transgenic genotype, localization of GFP is demonstrated in early and late stages of oogenesis: (A,B) vas+; (D,E) vas{Delta}616-618; (G,H) vas{Delta}616; (J,K) vas{Delta}617; (M,N) vas{Delta}618. Protein expression in ovaries of transgenic flies is demonstrated on western blots probed with anti-Vas. (C) Vas+; (F) Vas{Delta}616-618; (I) Vas{Delta}616; (L) Vas{Delta}617; (O) Vas{Delta}618. Flies contained two copies of each transgene in the wild-type background. For each sample, the faster migrating band represents endogenous Vas and the slower migrating band represents the transgenic GFP-Vas.





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