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Fig. 4. Vas-eIF5B interaction is required for the progression of oogenesis. (A) Western blot of ovarian extracts from wild-type (OreR), vasPH165, vasPH165;P{vas+} and vasPH165;P{vas{Delta}617} females was probed with anti-Vas. The same blot was probed with anti-4E-BP as a loading control. (B) Ovaries from vas PH165 females produce few mature stage 14 eggs owing to a developmental arrest during oogenesis. (C) Expression of a wild-type vas+ transgene rescues this defect of the vasPH165 mutation, allowing the production of abundant stage14 eggs. (D) Ovaries from vasPH165;P{vas{Delta}617} females resemble those of vasPH165, exhibiting a similar developmental arrest during oogenesis and producing few mature eggs.





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