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Fig. 8. Reduced Vas-eIF5B binding does not abrogate somatic patterning or Nos deployment. RNA in situ hybridization for ftz was used as an indicator of somatic segmentation at the cellular blastoderm stage. (A) Fifty percent of vas^PD;P{vas⁶¹⁷} embryos revealed a normal ftz distribution of seven transverse stripes. (B,C) In 20% of vas^PD;P{vas⁶¹⁷} embryos, stripes 4-6 were weaker and less defined than the others (B), and in 30%, more severe defects such as deletions and fusions of these segments were apparent (C). (D) Nos protein, visualized through immunostaining in 0- to 2-hour-old embryos, was present at the posterior of 95% of vas^PD;P{vas⁺} embryos. (E,F) Posterior Nos was detectable in 55% of the vas^PD;P{vas⁶¹⁷} embryos (E), and undetectable in the remaining 45% (F).

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