|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. (A-C) At stage 17, in the DD2-misexpressed limb, Meis2 was induced
(B) in an area where co-expressed EGFP proteins (shown in green) were detected
(A). Normal expression was shown in C. (D-F) At stage 19, similar but more
extensive induction of Meis2 was observed. (G,H) Implantation of
BMP4-soaked beads repressed Meis2 expression (red arrowheads) in both
a proximal area (G) and a distal end (H), with a deformity of the AER (H). (I)
Misexpression of DD2 cancelled the BMP4-mediated repression of Meis2,
with expansion of Meis2 expression to the distal end. (J) A
luciferase reporter plasmid containing 700 bp 5' region of human
MEIS1 was transfected into HepG2 cells, along with BMP4, Smad1,
Dach1, VP=Dach1 and mouse Sin3a expression plasmids. Expression of BMP4, Smad1
and Dach1 repressed this reporter. This repression was enhanced by the
addition of mouse Sin3a. By contrast, when VP=Dach1 was expressed, activation
of this reporter was obtained.
|
