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Fig. 3. Expression patterns of Bmp2 and Bmp4 in E3.5 canal pouches. (A) Arrows indicate the Bmp2-negative domain in the dorsal region of the canal pouch that will develop into the common crus. The ventral margins of the two Bmp2 expression domains (arrowheads in A) abut the Bmp4 sensory domains shown for another specimen in B. Arrowheads in B indicate Bmp4 expression in the mesenchyme. (C) Schematic showing the Bmp2 and Bmp4 expression domains superimposed. (D) Horizontal section through the lateral canal at E9. The Bmp2 hybridization signal is strong in the thick, outer rim, but weak in the thin, inner rim of the semicircular canals (arrowheads) and absent in the common crus (arrow). Orientations in C apply to A and B. Abbreviations are the same as for Fig. 1. AC, anterior crista; LC, lateral crista; lsc, lateral semicircular canal; PC, posterior crista. Scale bar in D: 100 µm.

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