|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Ectopic Notch activation expands a population of Ptf1-p48-positive,
amylase-negative cells in explant cultures of E10.5 dorsal pancreatic buds.
E10.5 dorsal epithelial buds were infected with lentiviral vectors encoding
either GFP alone or GFP and Hes1, then recombined with mesenchyme and allowed
to differentiate in vitro. (A,C,E) Deconvolution fluorescent microscopy
demonstrating patterns of Ptf1-p48 and amylase expression following infection
with GFP alone. (B,D,F) Expression patterns following infection with GFP;Hes1.
Note apical amylase expression in majority of Ptf1-p48 cells infected with GFP
alone, but expansion of a Ptf1a-p48-positive, amylase-negative population in
cells infected with GFP;Hes1. Broken lines in A and B indicate contour of
representative GFP-positive, Ptf1-p48-positive cells either expressing (A) or
lacking amylase (B). A similar pattern is observed following infection with
GFP;Notch1-IC. Scale bar: 10 µm.
|
