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Fig. 5. Notch pathway activation delays exocrine differentiation in developing
zebrafish pancreas. Heat-shocked embryos expressing a hsp:Gal4
transgene alone or in combination with UAS:notch1aICD were assessed
for initiation of ptf1a-p48 and trypsin expression, as well
as expression of insulin and pdx1. Following heat shock at
24 hpf, ptf1a-p48 expression is delayed in 32 hpf
hsp:Gal4;UAS:notch1aICD embryos (B) compared with hsp:Gal4
controls (A), but recovers by 34 hpf (data not shown). As assessed at 44 hpf,
both trypsin (E,F) and insulin (G,H) expression are reduced
in hsp:Gal4;UAS:notch1aICD embryos compared with hsp:Gal4
controls, even while ptf1a-p48 (C,D) and pdx1 (I,J)
expression remain normal. Following delayed heat shock initiated at 34 hpf
(after normal onset of ptf1a-p48 expression), ptf1a-p48
expression remains normal at all time points (M,N), whereas expression of
trypsin is delayed (K,L). Arrowheads in A,C,D,M,N indicate endodermal
domain of ptf1a-p48 expression, distinct from expression in
developing hindbrain. Arrowheads in I and J indicate pdx1-positive principal
islet; arrows indicate adjacent pdx1-positive intestine.
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